High resolution multiple excitation spot optical microscopy

High-resolution optical diffraction microscopy.

In an optical diffraction microscopy experiment, one measures the phase and amplitude of the field diffracted by the sample and uses an inversion algorithm to reconstruct its map of permittivity. We show that with an iterative procedure accounting for multiple scattering, it is possible to visualize details smaller than lambda/4 with relatively few illumination and observation angles. The roles...

Saturated patterned excitation microscopy--a concept for optical resolution improvement.

The resolution of optical microscopy is limited by the numerical aperture and the wavelength of light. Many strategies for improving resolution such as 4Pi and I5M have focused on an increase of the numerical aperture. Other approaches have based resolution improvement in fluorescence microscopy on the establishment of a nonlinear relationship between local excitation light intensity in the sam...

Electron beam excitation assisted optical microscope with ultra-high resolution.

We propose electron beam excitation assisted optical microscope, and demonstrated its resolution higher than 50 nm. In the microscope, a light source in a few nanometers size is excited by focused electron beam in a luminescent film. The microscope makes it possible to observe dynamic behavior of living biological specimens in various surroundings, such as air or liquids. Scan speed of the nano...

Optical-Resolution Photoacoustic Microscopy

HIGH - RESOLUTION , MULTIPLE OPTICAL MODE CONFOCAL MICROSCOPE : II . ThEORETICAL ASPECTS OF CONFOCAL TRANSMISSION MICROSCOPY

Much analysis of microscope image formation is based upon the so-called weak scaucring approximation in which an image is linearly related to an object by a convenient convolution property which greatly simplifies reconstruction. This approximation is often invalid in tmnsmission microscopy. A better model of transmission must account for multiple scauered components in the light distribution. ...